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Jubei Yagyu (柳生 十兵衛, Yagyū Jūbee) is a reoccuring character who was introduced in Samurai Shodown. He was dropped from the series after Samurai Shodown II but, like Tam Tam and Charlotte, returns to the series in Samurai Shodown IV. He is another interpretation of the often romanticized samurai, Yagyū Jūbei Mitsuyoshi. Like other mythical incarnations, he is the one-eyed master of Yagyū Shinkage-ryū and friends with Hanzo Hattori. According to a Gamest Mook, he was planned to appear in Samurai Shodown 64 but was taken out due to time constraints. Before Samurai Shodown: Warriors Rage debut, he was the only true samurai in the series.
In the RPG, he was an occasional guest member controlled by the computer and thus was unplayable.
In Samurai Shodown V, he teaches swordsmanship to the Tokugawa clan, with Yoshitora Tokugawa as his last disciple. He goes to retrieve the delinquent heir after Yoshitora's father (implied to be Tokugawa Ieharu) passes away. He decides to become a ronin (a lordless samurai) by the time Samurai Shodown takes place, abandoning the strict and regulated life of the dojo and Japanese nobility, to pursue personal enlightenment. Throughout the series, Jubei is hired by the shogunate to kill the demons and other possible threats that wander Japan.
His role in Shinsetsu Samurai Spirits: Bushidou Retsuden doubles as a sensei to his own dojo and an agent to the bakufu (the then Japanese government). He asks the party to further his investigation for him since he has to teach the lord's sons swordsmanship. In the second chapter, he suffers a mortal blow from one of Mizuki Rashojin's minions, Agon, and his spirit gets sucked into one of the Evil Bells.
Jubei is also the protagonist in Yagyu Kachou, an official side story set in an alternate reality where Samurai Shodown characters are placed in the modern world. In this story, he is a struggling salary man (standard Japanese business man) who has fallen on hard times. He's often picked on by various characters from the series, though Hanzo and Tam Tam are his closest friends.